plko 1 vector Search Results


c2c12 cells  (Addgene inc)
96
Addgene inc c2c12 cells
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
C2c12 Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c2c12 cells/product/Addgene inc
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c2c12 cells - by Bioz Stars, 2026-03
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plko.1-puro plasmid  (Merck KGaA)
90
Merck KGaA plko.1-puro plasmid
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Plko.1 Puro Plasmid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plko.1-puro plasmid/product/Merck KGaA
Average 90 stars, based on 1 article reviews
plko.1-puro plasmid - by Bioz Stars, 2026-03
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plko.1-based short hairpinrna(shrna) lentiviral vectors  (Biomics Biotechnologies)
90
Biomics Biotechnologies plko.1-based short hairpinrna(shrna) lentiviral vectors
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Plko.1 Based Short Hairpinrna(Shrna) Lentiviral Vectors, supplied by Biomics Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plko.1-based short hairpinrna(shrna) lentiviral vectors/product/Biomics Biotechnologies
Average 90 stars, based on 1 article reviews
plko.1-based short hairpinrna(shrna) lentiviral vectors - by Bioz Stars, 2026-03
90/100 stars
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lentiviral expression vector plko.1  (EuroClone)
90
EuroClone lentiviral expression vector plko.1
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Lentiviral Expression Vector Plko.1, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral expression vector plko.1/product/EuroClone
Average 90 stars, based on 1 article reviews
lentiviral expression vector plko.1 - by Bioz Stars, 2026-03
90/100 stars
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plko.1-puro vector  (Sigma-Genosys)
90
Sigma-Genosys plko.1-puro vector
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Plko.1 Puro Vector, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plko.1-puro vector/product/Sigma-Genosys
Average 90 stars, based on 1 article reviews
plko.1-puro vector - by Bioz Stars, 2026-03
90/100 stars
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hmox1 knockdown vectors plko.1-hmox1a/b/c  (Synbio Technologies LLC)
90
Synbio Technologies LLC hmox1 knockdown vectors plko.1-hmox1a/b/c
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Hmox1 Knockdown Vectors Plko.1 Hmox1a/B/C, supplied by Synbio Technologies LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hmox1 knockdown vectors plko.1-hmox1a/b/c/product/Synbio Technologies LLC
Average 90 stars, based on 1 article reviews
hmox1 knockdown vectors plko.1-hmox1a/b/c - by Bioz Stars, 2026-03
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plko.1-puro lentiviral shrna plasmid  (Fisher Scientific)
90
Fisher Scientific plko.1-puro lentiviral shrna plasmid
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Plko.1 Puro Lentiviral Shrna Plasmid, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plko.1-puro lentiviral shrna plasmid/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
plko.1-puro lentiviral shrna plasmid - by Bioz Stars, 2026-03
90/100 stars
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plko.1 vector  (Merck & Co)
90
Merck & Co plko.1 vector
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Plko.1 Vector, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plko.1 vector/product/Merck & Co
Average 90 stars, based on 1 article reviews
plko.1 vector - by Bioz Stars, 2026-03
90/100 stars
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lentiviral vectors containing single shrna or utx cds plko.1 vectors  (Illumina Inc)
90
Illumina Inc lentiviral vectors containing single shrna or utx cds plko.1 vectors
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Lentiviral Vectors Containing Single Shrna Or Utx Cds Plko.1 Vectors, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral vectors containing single shrna or utx cds plko.1 vectors/product/Illumina Inc
Average 90 stars, based on 1 article reviews
lentiviral vectors containing single shrna or utx cds plko.1 vectors - by Bioz Stars, 2026-03
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plko.1-puro[shrna] vectors  (FUJIFILM)
90
FUJIFILM plko.1-puro[shrna] vectors
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Plko.1 Puro[Shrna] Vectors, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plko.1-puro[shrna] vectors/product/FUJIFILM
Average 90 stars, based on 1 article reviews
plko.1-puro[shrna] vectors - by Bioz Stars, 2026-03
90/100 stars
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lentiviral vector plko.1-egfp  (BioVector Inc)
90
BioVector Inc lentiviral vector plko.1-egfp
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Lentiviral Vector Plko.1 Egfp, supplied by BioVector Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral vector plko.1-egfp/product/BioVector Inc
Average 90 stars, based on 1 article reviews
lentiviral vector plko.1-egfp - by Bioz Stars, 2026-03
90/100 stars
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tet-plko.1-puro vectors  (Novartis)
90
Novartis tet-plko.1-puro vectors
Changes in SphK1 and SPL during differentiation of <t>C2C12</t> cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.
Tet Plko.1 Puro Vectors, supplied by Novartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tet-plko.1-puro vectors/product/Novartis
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Image Search Results


Changes in SphK1 and SPL during differentiation of C2C12 cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.

Journal: The FASEB Journal

Article Title: Sphingosine phosphate lyase regulates myogenic differentiation via S1P receptor-mediated effects on myogenic microRNA expression

doi: 10.1096/fj.13-233155

Figure Lengend Snippet: Changes in SphK1 and SPL during differentiation of C2C12 cells. C2C12 cells were propagated under proliferation conditions until time 0, when the medium was replaced with differentiation medium containing 2% horse serum. Cells were harvested at d 0–5. Whole-cell extracts were evaluated by immunoblotting with antibodies specific for MHC, myogenin, SPL, SphK1 (SK1), and GAPDH loading control. A) Representative immunoblot. B) Quantification of autoradiogram. All bands were normalized to the loading control for the corresponding time point. Experiment is representative of 3 similar experiments.

Article Snippet: SPL knockdown (KD) in C2C12 cells The lentiviral vector pLKO.1 (Addgene plasmid 10878; Addgene, Cambridge, MA, USA) was used to clone small hairpin RNAs targeting the murine SPL gene Sgpl1 , according to pLKO.1 protocol ( http://www.addgene.com ), as we described for human SPL-KD cells ( 29 ).

Techniques: Western Blot

Generation of SPL-KD and vector control C2C12 lines. A) Phase-contrast images of C2C12 cells after lentiviral transduction to knock down murine SPL. Scale bar = 100 μm. B) SPL knockdown was confirmed by immunoblotting extracts of cells maintained in proliferation or differentiation conditions. Actin was used as a loading control. C) SPL enzyme activity. n = 3/group. SPL-specific activity is expressed as picomoles product formation per milligram protein per minute. *P < 0.05. D) Intracellular S1P concentration was determined by mass spectrometry and normalized to phospholipid content (PC). Data are presented as percentage control cell levels, n = 3/group. *P < 0.05. E) Extracellular S1P concentration was determined as in D; n = 4/group. Data are presented as percentage control levels. *P < 0.05. F) Expression of S1PR1–5 was compared in SPL-KD and control cells by qRT-PCR, with results normalized to GAPDH; n = 3/group. These experiments were performed ≥3 times with similar results. *P < 0.05.

Journal: The FASEB Journal

Article Title: Sphingosine phosphate lyase regulates myogenic differentiation via S1P receptor-mediated effects on myogenic microRNA expression

doi: 10.1096/fj.13-233155

Figure Lengend Snippet: Generation of SPL-KD and vector control C2C12 lines. A) Phase-contrast images of C2C12 cells after lentiviral transduction to knock down murine SPL. Scale bar = 100 μm. B) SPL knockdown was confirmed by immunoblotting extracts of cells maintained in proliferation or differentiation conditions. Actin was used as a loading control. C) SPL enzyme activity. n = 3/group. SPL-specific activity is expressed as picomoles product formation per milligram protein per minute. *P < 0.05. D) Intracellular S1P concentration was determined by mass spectrometry and normalized to phospholipid content (PC). Data are presented as percentage control cell levels, n = 3/group. *P < 0.05. E) Extracellular S1P concentration was determined as in D; n = 4/group. Data are presented as percentage control levels. *P < 0.05. F) Expression of S1PR1–5 was compared in SPL-KD and control cells by qRT-PCR, with results normalized to GAPDH; n = 3/group. These experiments were performed ≥3 times with similar results. *P < 0.05.

Article Snippet: SPL knockdown (KD) in C2C12 cells The lentiviral vector pLKO.1 (Addgene plasmid 10878; Addgene, Cambridge, MA, USA) was used to clone small hairpin RNAs targeting the murine SPL gene Sgpl1 , according to pLKO.1 protocol ( http://www.addgene.com ), as we described for human SPL-KD cells ( 29 ).

Techniques: Plasmid Preparation, Transduction, Western Blot, Activity Assay, Concentration Assay, Mass Spectrometry, Expressing, Quantitative RT-PCR